5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺 (CAS No.1360547-55-2)
- 中文别名
- 5'-HEX亚磷酰胺；HEX荧光标记亚磷酰胺
- 英文名称
- 5'-Hexachlorofluorescein Phosphoramidite
- 分子式
- C46H52Cl6N3O10P
- 分子量
- 1050.61（C46H52Cl6N3O10P）；
- 所属类别
- 试剂实验设备
- 物化性质
- 白色至淡黄色固体粉末，可溶于乙腈、二氯甲烷等有机溶剂，不溶于水。对湿气和酸敏感，应在干燥、避光、低温（-20°C）条件下储存。该化合物为亚磷酰胺类荧光标记单体，在标准DNA/RNA固相合成条件下可与寡核苷酸5'-羟基高效偶联。
- 外观
- 白色至淡黄色固体粉末；
- 纯度
- ≥98%（HPLC）；
- 溶解性
- 溶于乙腈、二氯甲烷等有机溶剂，不溶于水；
- 储存条件
- -20°C干燥避光保存，惰性气体保护。
English description（英文原文，共 1377 字符）
Product Name:5'-Hexachlorofluorescein Phosphoramidite
English Name:5'-Hexachlorofluorescein Phosphoramidite
CAS No.:1360547-55-2
Aliases:5'-HEX Amidite, HEX Phosphoramidite
Molecular Formula:C46H52Cl6N3O10P
Physical Properties:White to pale yellow solid powder, soluble in organic solvents such as acetonitrile and dichloromethane, insoluble in water. Sensitive to moisture and acid; store dry, protected from light, and at low temperature (-20°C). As a fluorescent labeling phosphoramidite monomer, it couples efficiently with the 5'-hydroxyl group of oligonucleotides under standard solid-phase DNA/RNA synthesis conditions.
Uses:Used for fluorescent labeling at the 5'-end during solid-phase oligonucleotide synthesis, introducing hexachlorofluorescein (HEX) into DNA or RNA sequences. Widely applied in fluorescence in situ hybridization (FISH), real-time quantitative PCR (qPCR), DNA sequencing, and gene expression analysis. Its excitation/emission wavelengths (approximately 535 nm/553 nm) are suitable for multiplex fluorescence detection systems.
Technical Specifications:
Appearance: white to pale yellow solid powder;
Purity: ≥98% (HPLC);
Molecular weight: 1050.61 (C46H52Cl6N3O10P);
Solubility: soluble in acetonitrile, dichloromethane and other organic solvents, insoluble in water;
Storage: keep dry and protected from light at -20°C, under inert gas.
English Name:5'-Hexachlorofluorescein Phosphoramidite
CAS No.:1360547-55-2
Aliases:5'-HEX Amidite, HEX Phosphoramidite
Molecular Formula:C46H52Cl6N3O10P
Physical Properties:White to pale yellow solid powder, soluble in organic solvents such as acetonitrile and dichloromethane, insoluble in water. Sensitive to moisture and acid; store dry, protected from light, and at low temperature (-20°C). As a fluorescent labeling phosphoramidite monomer, it couples efficiently with the 5'-hydroxyl group of oligonucleotides under standard solid-phase DNA/RNA synthesis conditions.
Uses:Used for fluorescent labeling at the 5'-end during solid-phase oligonucleotide synthesis, introducing hexachlorofluorescein (HEX) into DNA or RNA sequences. Widely applied in fluorescence in situ hybridization (FISH), real-time quantitative PCR (qPCR), DNA sequencing, and gene expression analysis. Its excitation/emission wavelengths (approximately 535 nm/553 nm) are suitable for multiplex fluorescence detection systems.
Technical Specifications:
Appearance: white to pale yellow solid powder;
Purity: ≥98% (HPLC);
Molecular weight: 1050.61 (C46H52Cl6N3O10P);
Solubility: soluble in acetonitrile, dichloromethane and other organic solvents, insoluble in water;
Storage: keep dry and protected from light at -20°C, under inert gas.
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常见问题
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺的 CAS 号是多少？
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺的 CAS 号是 1360547-55-2。
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺的分子式和分子量是什么？
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺的分子式为 C46H52Cl6N3O10P，分子量约 1050.61（C46H52Cl6N3O10P）；。
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺的英文名称是什么？
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺的英文名称为 5'-Hexachlorofluorescein Phosphoramidite。
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺还有哪些别名？
- 5'-六氯荧光素氨基磷酸酯 OR 5'-HEX,亚磷酰胺又称 5'-HEX亚磷酰胺、HEX荧光标记亚磷酰胺。
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