Protein G亲和层析树脂 (CAS No.None)
- 物化性质
- 该树脂以高交联琼脂糖为基质,偶联重组Protein G,呈球形颗粒,粒径约45-165 μm。具有良好的亲水性和化学稳定性,可耐受常用的层析洗脱条件。在pH 3-9范围内稳定,推荐储存于20%乙醇溶液中,2-8℃保存。
- 储存条件
- 20%乙醇,2-8℃
English description(英文原文,共 1206 字符)
Product Name:Protein G Affinity Chromatography Resin
English Name:Protein G Affinity Chromatography Resin
Aliases:Recombinant Protein G Agarose Gel, Protein G Agarose Resin, Antibody Purification Resin
Physical Properties:The resin is based on highly cross-linked agarose coupled with recombinant Protein G, in spherical beads with a particle size of approximately 45-165 μm. It exhibits good hydrophilicity and chemical stability, compatible with common chromatographic elution conditions. Stable in the pH range of 3-9, and is recommended for storage in 20% ethanol at 2-8°C.
Uses:Mainly used for affinity purification of antibodies. It specifically binds to the Fc region of immunoglobulin G (IgG), enabling efficient separation and purification of monoclonal and polyclonal antibodies. Suitable for extraction of IgG from cell culture supernatant, ascites fluid, or serum, as well as for immunoprecipitation and protein interaction studies.
Technical Specifications:
Ligand density: approximately 5 mg Protein G/mL resin
Dynamic binding capacity: ≥25 mg human IgG/mL resin
Particle size: 45-165 μm
pH stability: 3-9 (working), 2-11 (short-term cleaning)
Storage conditions: 20% ethanol, 2-8°C
English Name:Protein G Affinity Chromatography Resin
Aliases:Recombinant Protein G Agarose Gel, Protein G Agarose Resin, Antibody Purification Resin
Physical Properties:The resin is based on highly cross-linked agarose coupled with recombinant Protein G, in spherical beads with a particle size of approximately 45-165 μm. It exhibits good hydrophilicity and chemical stability, compatible with common chromatographic elution conditions. Stable in the pH range of 3-9, and is recommended for storage in 20% ethanol at 2-8°C.
Uses:Mainly used for affinity purification of antibodies. It specifically binds to the Fc region of immunoglobulin G (IgG), enabling efficient separation and purification of monoclonal and polyclonal antibodies. Suitable for extraction of IgG from cell culture supernatant, ascites fluid, or serum, as well as for immunoprecipitation and protein interaction studies.
Technical Specifications:
Ligand density: approximately 5 mg Protein G/mL resin
Dynamic binding capacity: ≥25 mg human IgG/mL resin
Particle size: 45-165 μm
pH stability: 3-9 (working), 2-11 (short-term cleaning)
Storage conditions: 20% ethanol, 2-8°C
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常见问题
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- Protein G亲和层析树脂的英文名称为 Protein G Affinity Chromatography Resin。
- Protein G亲和层析树脂还有哪些别名?
- Protein G亲和层析树脂又称 重组蛋白G琼脂糖凝胶、Protein G琼脂糖树脂、抗体纯化树脂。
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