磺基琥珀酰亚胺-6-[3-(2-吡啶基二硫代)丙酰胺]己酸 (CAS No.169751-10-4)

Product Name:Sulfosuccinimidyl-6-[3-(2-pyridyldithio)propionamido]hexanoate

English Name:Sulfosuccinimidyl-6-[3-(2-pyridyldithio)propionamido]hexanoate

CAS No.:169751-10-4

Aliases:Sulfo-LC-SPDP, Sulfo-LC-SPDP Crosslinker, Sulfosuccinimidyl-6-[3-(2-pyridyldithio)propionamido]hexanoate sodium salt

Molecular Formula:C18H22N3NaO8S3

Physical Properties:White to off-white solid. The sodium sulfonate group confers good water solubility, allowing direct use in aqueous buffers. The compound contains a 2-pyridyldisulfide bond, which can be cleaved by reducing agents such as DTT. The NHS ester is moisture-sensitive and prone to hydrolysis.

Uses:Sulfo-LC-SPDP is a water-soluble heterobifunctional crosslinker used for biomolecule conjugation and surface modification. The NHS ester reacts with primary amines at near-neutral pH (7–9) to form stable amide bonds, while the pyridyldithio group reacts with sulfhydryl groups at pH 5–7 to form cleavable disulfide bonds. It is widely used for protein–protein conjugation, protein–peptide crosslinking, cell surface labeling, membrane protein research, enzyme coupling, and antibody modification.

Technical Specifications:

Purity: ≥99% (HPLC)

Molecular weight: 527.6

Solubility: readily soluble in water, DMSO, and DMF

Storage: keep at -20 °C, protected from light and moisture

Package: 1 g per bottle