Phenylalanine, N-[N,N-bis(carboxymethyl)glycyl]- (CAS No.93583-09-6)

Product Name:Phenylalanine, N-[N,N-bis(carboxymethyl)glycyl]-

English Name:Phenylalanine, N-[N,N-bis(carboxymethyl)glycyl]-

CAS No.:93583-09-6

Aliases:Phe-NTA, N-[[bis(carboxymethyl)amino]acetyl]phenylalanine

Molecular Formula:C15H18N2O7

Physical Properties:White to off-white powder. The molecule contains three dissociable carboxyl groups and one tertiary amine nitrogen, showing strong negative charge under physiological pH conditions. It is readily soluble in water and alkaline buffer solutions, and slightly soluble in polar organic solvents such as methanol and ethanol. The N,N-bis(carboxymethyl)glycyl moiety forms an IDA-like tridentate coordination structure and binds transition metal ions including Cu²⁺, Ni²⁺, Co²⁺, and Zn²⁺ to give stable chelates.

Uses:Primarily used as an IDA-like biomimetic ligand in immobilized metal affinity chromatography (IMAC); it chelates transition metal ions such as Cu²⁺, Ni²⁺, Co²⁺, and Zn²⁺ for the purification and separation of proteins, especially His-tagged proteins. It is also applicable to the preparation of metal-chelated enzyme immobilization carriers, chelation and removal of heavy metal ions (Pb²⁺, Cd²⁺) in environmental remediation and industrial water treatment, and as a chelating ligand precursor for metal radionuclides (⁶⁴Cu, ⁶⁸Ga, ¹¹¹In) in molecular imaging probes and biomaterial surface modification.

Technical Specifications:

Purity: ≥95%

Molecular weight: 338.32

Appearance: white to off-white powder

Solubility: readily soluble in water and alkaline buffer solutions; slightly soluble in methanol and ethanol

Storage: keep sealed and protected from light at 2–8°C